NEK1 Rabbit Polyclonal Antibody

SKU: orb100828

Description

NEK1 Rabbit Polyclonal Antibody

Research Area

Cell Biology, DNA Damage / RNA Repair, Epigenetics and Nuclear Signaling, Kinases, Phosphatases

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NEK1 (301-400/1258aa)
• Target: NEK1
• Molecular Weight: 143 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEK1; NEK1_HUMAN; Never in mitosis A-related kinase 1; NIMA(never in mitosis gene a)-related kinase 1; NIMA-related kinase 1; NimA-related protein kinase 1; NY-REN-55; Renal carcinoma antigen NY-REN-55; Serine/threonine-protein kinase Nek1; SRPS2; DKFZp686D06121; DKFZp686K12169; KIAA1901; MGC138800.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (NEK1) Polyclonal Antibody, Unconjugated (orb100828) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Quick Database Links

Gene Symbol

NEK1

UniProt

Q96PY6