Anti-NEFM Antibody

• Catalog Number: orb1289733
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to NEFM
• Target: NEFM
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human of human NEFM. The exact sequence is proprietary.
• UniProt ID: P12839, P08553, P07197
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NEF3; NFM; Neurofilament medium polypeptide; NF-M;
• Note: For research use only
• Entrez: 18040, 24588, 4741

Western blot analysis of NEFM expression in SHSY5Y (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)

Immunofluorescent analysis of NEFM staining in mouse brain. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.