NF-M Rabbit Polyclonal Antibody (APC)

• Catalog Number: orb995376
• Category: Antibodies
• Description: NF-M Rabbit Polyclonal Antibody (APC)
• Target: Nefm
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: APC
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Human, Porcine
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human NF-M (101-200/916aa)
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Neurofilament medium polypeptide; 160 kDa neurofil
• Note: For research use only

Blank control: 293T. Primary Antibody (green line): Rabbit Anti-NEFM antibody (orb995376), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.