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Anti-MTA1 Antibody

Catalog Number: orb393115

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb393115
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MTA1.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human MTA1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500:1000, IHC-P: 1:100:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMTA1
Entrez9112, 64520, 116870
UniProt IDQ8K4B0, Q62599, Q13330
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesMetastasis-associated protein MTA1
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-MTA1 Antibody

Western blot analysis of MTA1 expression in HEK293T (A), Hela (B), HGC27 (C) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 90 kD)

Anti-MTA1 Antibody

Immunohistochemical analysis of MTA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-MTA1 Antibody

Immunofluorescent analysis of MTA1 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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