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MTA1 Rabbit Polyclonal Antibody

Catalog Number: orb11044

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11044
CategoryAntibodies
DescriptionMTA1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human MTA1 (601-715/715aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW81 kDa
TargetMTA1
UniProt IDQ13330
RRIDAB_10752950
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMetastasis-associated 1; Metastasis associated 1;
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NoteFor research use only
Expiration Date12 months from date of receipt.
MTA1 Rabbit Polyclonal Antibody

Blank control (black line): SH-SY5Y. Primary Antibody (green line): Rabbit Anti-MTA1 antibody (orb11044), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MTA1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (orb11044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

MTA1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (orb11044) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

MTA1 Rabbit Polyclonal Antibody

Sample: Lung (Mouse) Lysate at 30 ug, Primary: Anti-MTA1 (orb11044) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution, Predicted band size: 81 kD, Observed band size: 85 kD.

MTA1 Rabbit Polyclonal Antibody

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated (orb11044) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

MTA1 Rabbit Polyclonal Antibody

Tissue/Cell: transplanted tumor of cervical carcinoma in nude mice, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated (orb11044) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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