Anti-MSH6 Antibody

• Catalog Number: orb705455
• Category: Antibodies
• Description: Mouse monoclonal antibody to MSH6
• Target: MSH6
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human MSH6. The exact sequence is proprietary.
• UniProt ID: P52701
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300, IF: 1:100-1:500
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-GTBP antibody, anti-DNA mismatch repair prote
• Note: For research use only
• Entrez: 2956

Western blot analysis of MSH6 expression in Hela (A), HEK293 (B), HepG2 (C) whole cell lysates. (Predicted band size: 152 kD; Observed band size: 180 kD)

Immunohistochemical analysis of MSH6 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of MSH6 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.