MSH6 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1173059
• Category: Antibodies
• Description: MSH6 Recombinant Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: H3A4
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG2a/Kappa
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=0, Flow-Cyt=1:20-1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MSH6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Lysate: Jurkat, Protein loading quantity: 20 µg, Exposure time: 180 s, Predicted MW: 152 kDa, Observed MW: 170 kDa.

Cell line: MCF-7, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab dilution/color: 1:100/Red, Unlabelled control/color: Cell without incubation with primary antibody and secondary antibody/Black, Isotype control/color: Rabbit monoclonal IgG/Blue, Comment: Color red is the positive signal for orb1173059.

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1173059.

Tissue: Human colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1173059.