CD8a Antibody

SKU: orb305473

Description

Rat monoclonal antibody to CD8a

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Mouse
• Application Notes:
  Flow cytometry: Recommended dilution: 1.5 μg/ml. Immunohistochemistry (frozen sections): Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone No.: 53-6.7
• Immunogen: Murine spleen cells
• Target: CD8a
• Purification: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ly-2, Ly-B, Ly-35, Lyt-2, BB154331

Separation of murine CD8a positive splenocytes (red-filled) from myeloid cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (concentration in sample 3 μg/ml) DAR PE.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (concentration in sample 3 μg/ml) DAR PE.

Quick Database Links

Gene Symbol

CD8a

UniProt

P01731

NCBI Gene ID

12525