CD8a Antibody

SKU: orb222736

Description

Rat monoclonal antibody to CD8

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FA, FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Mouse
• Application Notes:
  Functional application: Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation. Flow cytometry: Recommended dilution: 1.5 μg/ml. Immunohistochemistry (frozen sections): Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone No.: 53-6.7
• Immunogen: Murine spleen cells
• Target: CD8a
• Purification: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ly-2, Ly-B, Ly-35, Lyt-2, BB154331

Quick Database Links

Gene Symbol

CD8a

UniProt

P01731

NCBI Gene ID

12525