CD154 Antibody (FITC)

SKU: orb44603

Description

Hamster Monoclonal to CD154.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Mouse
• Application Notes:
  Flow cytometry: Recommended dilution: 2-3 μg/ml; positive control: 6-8 hour activated murine splenocytes.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG1
• Clone No.: MR-1
• Immunogen: Activated mouse Th1 clone D1.6
• Target: CD154
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

Flow cytometry multicolor surface staining pattern of murine stimulated (PMA + Ionomycin) lymphocytes using anti-mouse CD154 (MR-1) FITC antibody (concentration in sample 1 μg/ml) and anti-mouse CD3 (145-2C11) APC antibody (concentration in sample 2 μg/ml).

Separation of murine CD154 positive CD3 positive lymphocytes (red-filled) from CD154 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine stimulated (PMA + Ionomycin) splenocyte suspension stained using anti-mouse CD154 (MR-1) FITC antibody (concentration in sample 1 μg/ml).

Flow cytometry surface staining pattern of murine stimulated (PMA + Ionomycin) splenocyte suspension stained using anti-mouse CD154 (MR-1) FITC antibody (concentration in sample 1 μg/ml).

Quick Database Links

Gene Symbol

CD154

UniProt

P29965

NCBI Gene ID

959