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Anti-Hu CD154 FITC
Catalog Number: orb421975
| Catalog Number | orb421975 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD154 conjugated to FITC |
| Clonality | Monoclonal |
| Clone Number | 24-31 |
| Tested applications | FC |
| Reactivity | Human, Primate |
| Isotype | Mouse IgG1 |
| Immunogen | human CD154 fusion protein |
| Antibody Type | Primary Antibody |
| Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | FITC |
| Target | CD154 |
| Entrez | 21947 |
| UniProt ID | P27548 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-CD40L antibody, anti-CD40 ligand antibody, an Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry detection of CD154 / CD40L in IgE-activated human peripheral blood with anti-CD154 / CD40L (24-31) FITC. Orange = blank, blue = CD154 negative cells, red = CD154 positive cells.
Separation of PMA/Ionomycine activated PBMCs stained using anti-human CD154 (24-31) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from PMA/Ionomycine activated PBMCs stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 3 μg/ml, same as CD154 FITC concentration, black-dashed) in flow cytometry analysis (surface staining) of PMA/Ionomycine activated PBMCs.
