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Anti-MMP1 Antibody

Catalog Number: orb214254

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214254
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MMP1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Primate, Rabbit, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MMP1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMMP1
Entrez4312
UniProt IDP81563, P03956
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti CLG antibody, anti Interstitial collagenase a
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-MMP1 Antibody

Western blot analysis of MMP1 expression in HEK293T (A), Hela (B), A375 (C) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 52 kD)

Anti-MMP1 Antibody

Immunohistochemical analysis of MMP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-MMP1 Antibody

Immunofluorescent analysis of MMP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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