MLKL Rabbit Monoclonal Antibody

• Catalog Number: orb866681
• Category: Antibodies
• Description: Anti-MLKL Rabbit Monoclonal Antibody. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from MLKL
• UniProt ID: Q8NB16
• MW: 54 kDa
• Tested applications: IP, WB
• Application notes: WB 1:500-2000IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: 26M37
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Mixed lineage kinase domain-like protein; hMLKL; M
• Note: For research use only

Western blot analysis of MLKL using anti-MLKL antibody (orb866681). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HeLa whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MLKL antigen affinity purified monoclonal antibody (Catalog # orb866681) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for MLKL at approximately 54 kDa. The expected band size for MLKL is at 54 kDa.