Phospho-MLKL (Ser345) Recombinant Rabbit Monoclonal Antibody

SKU: orb608076

Description

Phospho-MLKL (Ser345) Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Research, Kinases, Necroptosis Pathway, SARS /COVID-19

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB=1:500-2000, IP=1:10-50, ICC/IF=1:50-200
• Reactivity: Mouse
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 7G4
• Immunogen: A synthesized peptide derived from mouse Mlkl around the phosphorylation site of S345 QN-pS-IS
• Target: MLKL
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

phospho-MLKL(S345); p-MLKL(S345); MLKL(p-S345); hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain-like protein; mixed lineage kinase domain like pseudokinase; MLKL_HUMAN.

Western blot analysis of Phospho-MLKL (S345) on L929 cell lysates. Lane 1: L929 cells, whole cell lysate, 10 µg/lane. Lane 2: L929 cells were treated with 20 uM Z-VAD for 30 minutes, then added 20 ng/ml mTNF-alpha and 100 nM SM-164 for 4 hours, whole cell lysates, 10 µg/lane.

Quick Database Links

Gene Symbol

MLKL

UniProt

Q9D2Y4