Anti-MLH1 Antibody

• Catalog Number: orb1474614
• Category: Antibodies
• Description: Rabbit monoclonal antibody to MLH1
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide of human MLH1
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: MLH1
• Entrez: 4292, 17350, 81685
• UniProt ID: Q9JK91, P97679, P40692
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: COCA2; DNA mismatch repair protein Mlh1; MutL prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MLH1 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 85 kD)

Immunofluorescent analysis of MLH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.