MLH1 Antibody / MutL Homolog 1

• Catalog Number: orb1151494
• Category: Antibodies
• Description: Rabbit monoclonal antibody to MLH1
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MLH1/6284R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant full-length human MLH1 protein was used as the immunogen for the recombinant MLH1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant MLH1 antibody is available for research use only.
• UniProt ID: P40692
• Storage: Aliquot the recombinant MLH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant MLH1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma tissue with recombinant MLH1 antibody (clone MLH1/6284R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE Lynch Syndrome / Hereditary Non-Polyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (clone MLH1/6284R) at 2 ug/ml in PBS, 30 min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Hereditary Nonpolyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (MLH1/6284R) at 2 ug/ml.