MLH1 Antibody

SKU: orb1474256

Description

The MLH1 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Disclaimer: For research use only

Alternative Names

COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1

Western blot analysis of MLH1 expression in Hela (A), Jurkat (B), 293T (C), SW480 (D) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 85 kD)

Immunofluorescent analysis of MLH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P40692

NCBI Gene ID

4292