Anti-MLH1 Antibody

• Catalog Number: orb705404
• Category: Antibodies
• Description: Mouse monoclonal antibody to MLH1
• Target: MLH1
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human MLH1. The exact sequence is proprietary.
• UniProt ID: P40692
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300, IF: 1:100-1:500
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-COCA2 antibody, anti-DNA mismatch repair prot
• Note: For research use only
• Entrez: 4292

Western blot analysis of MLH1 expression in K562 (A), HEK293 (B), Hela (C) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 88 kD)

Immunohistochemical analysis of MLH1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of MLH1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.