Anti-Mesothelin [K1]

SKU: orb1821641

This product can be custom made

Description

Research Area

Cancer Biology

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, IHC, RIA, SBR, WB
Reactivity	Human, Monkey

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	K1
Immunogen	The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.
Target	Mesothelin
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow cytometry using the Anti-Mesothelin antibody K1. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of K1 (orb1821641, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with Anti-Mesothelin K1. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG1 version of K1 (orb1821641) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1821641, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.