Anti-Mesothelin [K1]

• Catalog Number: orb1821640
• Category: Antibodies
• Description: Anti-Mesothelin [K1]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: K1
• Tested applications: ELISA, IF, IHC, RIA, SBR, WB
• Reactivity: Human, Monkey
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: Mesothelin
• UniProt ID: Q13421
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentia
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-Mesothelin antibody K1. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of K1 (orb1821641, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with Anti-Mesothelin K1. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG1 version of K1 (orb1821641) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1821641, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.