Anti-MED25 Antibody

• Catalog Number: orb667258
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MED25
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human MED25. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MED25
• Entrez: 81857, 75613
• UniProt ID: Q71SY5, Q8VCB2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-ACID1 antibody, anti-ARC92 antibody, anti-PTO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MED25 expression in A549 (A), mouse brain (B), rat kidney (C) whole cell lysates. (Predicted band size: 55; 76; 78; 82; 84 kD; Observed band size: 78 kD)

Immunofluorescent analysis of MED25 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.