MCH Receptor 1 Antibody

• Catalog Number: orb665821
• Category: Antibodies
• Description: The MCH Receptor 1 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MCH Receptor 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MCH Receptor 1. The exact sequence is proprietary.
• UniProt ID: Q969V1
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1-500-1000, IF: 1-100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPR145; SLT; Melanin-concentrating hormone recepto
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 84539

Western blot analysis of MCH Receptor 1 expression in rat testis (A), BV2 (B), LOVO (C), HCT116 (D) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)

Immunohistochemical analysis of MCH Receptor 1 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MCH Receptor 1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.