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Anti-MCH Receptor 1 Antibody

Catalog Number: orb665821

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb665821
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MCH Receptor 1
TargetMCHR2
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MCH Receptor 1. The exact sequence is proprietary.
UniProt IDQ969V1
Tested applicationsIF, IH, WB
Dilution rangeWB: 1-500-1000, IF: 1-100-500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-GPR145 antibody, anti-SLT antibody, anti-Mela
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NoteFor research use only
Entrez84539
Anti-MCH Receptor 1 Antibody

Western blot analysis of MCH Receptor 1 expression in rat testis (A), BV2 (B), LOVO (C), HCT116 (D) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)

Anti-MCH Receptor 1 Antibody

Immunohistochemical analysis of MCH Receptor 1 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-MCH Receptor 1 Antibody

Immunofluorescent analysis of MCH Receptor 1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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