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Item 1 of 4
MCHR1 Antibody (C-term)
Catalog Number: orb1938790
| Catalog Number | orb1938790 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB28532 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 38940 Da |
| Target | This MCHR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-353 amino acids from the C-terminal region of human MCHR1. |
| UniProt ID | Q99705 |
| NCBI | NP_005288.3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Melanin-concentrating hormone receptor 1, MCH rece Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-MCHR1 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
MCHR1 Antibody (C-term) western blot analysis in CEM, K562 cell line lysates (35 ug/lane). This demonstrates the MCHR1 antibody detected the MCHR1 protein (arrow).
MCHR1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MCHR1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MCHR1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
