MCHR1 Antibody (C-term)

SKU: orb1938790

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB28532
• Immunogen: This MCHR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-353 amino acids from the C-terminal region of human MCHR1. Antigen Region: 342-353 aa.
• Target: MCHR1 (HGNC:4479)
• Molecular Weight: 38940 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Melanin-concentrating hormone receptor 1, MCH receptor 1, MCH-R1, MCHR-1, G-protein coupled receptor 24, MCH-1R, MCH1R, MCHR, SLC-1, Somatostatin receptor-like protein, MCHR1, GPR24, SLC1

Anti-MCHR1 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

MCHR1 Antibody (C-term) western blot analysis in CEM, K562 cell line lysates (35 ug/lane). This demonstrates the MCHR1 antibody detected the MCHR1 protein (arrow).

MCHR1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MCHR1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MCHR1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

MCHR1 (HGNC:4479)

UniProt

Q99705

RefSeq (Protein):NP_005288.3