MAP2 Mouse Monoclonal Antibody

SKU: orb1474020

Description

The MAP2 Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human MAP2. The exact sequence is proprietary.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human MAP2. The exact sequence is proprietary.
• Target: MAP2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: crude ascites with 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microtubule-associated protein 2; MAP-2

Western blot analysis of MAP2 expression in MCF7 (A) whole cell lysates. (Predicted band size: 199 kD; Observed band size: 56 kD)

Immunohistochemical analysis of MAP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MAP2 staining in brain tissue cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

MAP2

UniProt

P11137

NCBI Gene ID

4133