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Item 1 of 4
Anti-LCK Purified
Catalog Number: orb43898
| Catalog Number | orb43898 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Lck. |
| Clonality | Monoclonal |
| Clone Number | LCK-01 |
| Tested applications | FC, ICC, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Peptide corresponding to amino acids 22-36 in the sequence of human Lck. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | LCK |
| Entrez | 3932 |
| UniProt ID | P06239 |
| RRID | AB_10991164 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-Lck antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck; Lane 2: HEK-293T cell line transfected with Lck; Lane 3: HEK-293T cell line (non-transfected).
Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LCK (LCK-01) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD3 positive LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LCK (LCK-01) purified antibody (concentration in sample 9 µg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LCK (LCK-01) purified antibody (concentration in sample 9 µg/ml, GAM APC).
