SQSTM1/p62 Rabbit Polyclonal Antibody

• Catalog Number: orb704394
• Category: Antibodies
• Description: SQSTM1/p62 Rabbit Polyclonal Antibody
• Target: SQSTM1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human SQSTM1/p62 (1-440aa)
• UniProt ID: Q13501
• MW: 38/47 kDa
• Tested applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500, ICC/IF=1:100-500, Flow-Cyt=1:100-200, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SQSTM_HUMAN; Sequestosome-1; ORCA; OSIL; EBI3-asso
• Research Area: Autophagy, Cell Cycle, Epigenetics and Nuclear Sig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human appendix), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (orb704394) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (orb704394) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (orb704394) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: SQSTM1 / p62 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-SQSTM1 / p62 (orb704394) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Spleen (Mouse) Lysate at 40 ug, Lane 5: Muscle (Mouse) Lysate at 40 ug, Lane 6: Small intestine (Rat) Lysate at 40 ug, Lane 7: Hela (Human) Cell Lysate at 30 ug, Lane 8: A549 (Human) Cell Lysate at 30 ug, Lane 9: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-SQSTM1/p62 (orb704394) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.