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Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

Catalog Number: orb402531

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb402531
CategoryAntibodies
DescriptionAnti-Laminin/Lamc1/Lamc2/Lamc3 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenPeptide mixture of laminin gamma1,2,3 (NKLNEIEGSLNKAKDEMKAS; DLEERVRRQRNHLHLLETSI; LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW150, 220-250 kDa
UniProt IDP02468
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesLaminin subunit gamma-1; Laminin B2 chain; Laminin
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Immunofluorescence, 2μg/ml, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IF analysis of Laminin using anti-Laminin antibody. Laminin was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Laminin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IF analysis of Laminin using anti-Laminin antibody. Laminin was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Laminin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IF analysis of Laminin using anti-Laminin antibody. Laminin was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Laminin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

Western blot analysis of Laminin using anti-Laminin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Laminin at approximately 150, 220-250 kDa. The expected band size for Laminin is at 177 kDa.

  • Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody [orb215896]

    IHC,  IHC-Fr,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody [orb2623542]

    IHC,  IHC-Fr,  WB

    Human

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody [orb2623543]

    IHC,  IHC-Fr,  WB

    Human

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody [orb2623544]

    IHC,  IHC-Fr,  WB

    Human

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody [orb2623545]

    IHC,  IHC-Fr,  WB

    Human

    Rabbit

    Polyclonal

    HRP

    100 μg