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Catalog Number | orb340922 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to LBR |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human Lamin B Receptor |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1:2000, IHC-P: 1:50-1:200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | LBR |
Entrez | 89789, 3930, 98386 |
UniProt ID | Q14739, Q3U9G9, O08984 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Lamin-B receptor; Integral nuclear envelope inner Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Lamin B Receptor expression in K562 (A), HT29 (B), mouse spleen (C), mouse lung (D) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)
Immunofluorescent analysis of Lamin B Receptor staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, IP, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
iFluor647 |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE |