Cart summary

You have no items in your shopping cart.

Lamin B Receptor Rabbit Polyclonal Antibody

Catalog Number: orb5557

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5557
CategoryAntibodies
DescriptionLamin B Receptor Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, WB
Predicted ReactivityBovine, Equine, Rabbit, Rat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Lamin B Receptor (1-100/615aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, ICC/IF=1:25, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW68 kDa
TargetLBR
UniProt IDQ14739
RRIDAB_10930252
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesLBR_HUMAN; LMN2R; PHA; DHCR 14B; DHCR14B antibody
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Lamin B Receptor Rabbit Polyclonal Antibody

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Lamin B Receptor antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Lamin B Receptor Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Lamin B Receptor) polyclonal Antibody, Unconjugated (orb5557) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Lamin B Receptor Rabbit Polyclonal Antibody

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Lamin B Receptor (orb5557) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.

Lamin B Receptor Rabbit Polyclonal Antibody

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Lamin B Receptor (orb5557) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.

  • Anti-Lamin B Receptor Antibody [orb340922]

    IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • HP1-gamma (M) Antibody, Rabbit Polyclonal [orb66781]

    WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Lamin B Receptor Rabbit Polyclonal Antibody (HRP) [orb480275]

    WB

    Bovine, Equine, Rabbit, Rat

    Human, Mouse

    Rabbit

    Polyclonal

    HRP

    100 μl
  • Lamin B Receptor Rabbit Polyclonal Antibody (Biotin) [orb447151]

    FC,  ICC,  WB

    Bovine, Equine, Rabbit, Rat

    Human, Mouse

    Rabbit

    Polyclonal

    Biotin

    100 μl
  • Lamin B Receptor Rabbit Polyclonal Antibody (AP) [orb896700]

    WB

    Bovine, Equine, Rabbit, Rat

    Human, Mouse

    Rabbit

    Polyclonal

    AP

    100 μl