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Anti-KNP-I Antibody

Catalog Number: orb377957

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb377957
CategoryAntibodies
DescriptionRabbit polyclonal antibody to C21orf33.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human KNP-I
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000, IF/ICC: 1:10-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetC21orf33
Entrez8209, 102724023
UniProt IDP30042
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesHES1; KNPI; ES1 protein homolog mitochondrial; Pro
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-KNP-I Antibody

Western blot analysis of KNP-I expression in SW480 (A), HepG2 (B), mouse skin (C), mouse heart (D), rat skeletal muscle (E) whole cell lysates. (Predicted band size: 24; 28 kD; Observed band size: 25 kD)

Anti-KNP-I Antibody

Immunofluorescent analysis of KNP-I staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.