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Catalog Number | orb156005 |
---|---|
Category | Antibodies |
Description | C21orf33 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Canine, Gallus, Mouse, Porcine |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human C21orf33 (101-200/268aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 28 kDa |
Target | GATD3 |
UniProt ID | P30042 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Chromosome 21 open reading frame 33; D21S2048E; ES Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA (green).
Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-C21orf33 (orb156005) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.
Tissue/Cell: Rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated (orb156005) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Equine, Guinea pig, Mouse, Rat, Sheep, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Equine, Guinea pig, Human, Mouse, Rat, Sheep, Zebrafish | |
Rabbit | |
Polyclonal | |
HRP |
IHC, WB | |
Bovine, Equine, Guinea pig, Human, Mouse, Rat, Sheep, Zebrafish | |
Rabbit | |
Polyclonal | |
FITC |