Anti-JAM-A [6F4]

SKU: orb2645588

Description

Anti-JAM-A [6F4]

Research Area

Cancer Biology, Cell Biology

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, IF, IHC, WB
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	6F4
Immunogen	The original antibody was generated by immunizing BALB/c mice with MCF-7 cells. The humanized version was generated by grafting CDRs of parental mouse antibody onto human framework regions.
Target	JAM-A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD321; JAM-1; JAM1; JCAM; F11R; PAM-1; Junctional adhesion molecule A; Junctional adhesion molecule 1; Platelet F11 receptor

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunofluorescence staining of MCF7 cells with anti-JAM-A antibody 6F4. Immunofluorescence analysis of paraformaldehyde-fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 6F4 (orb2645587) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell junction staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645587, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-JAM-A antibody 6F4. Caco-2 cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of 6F4 (orb2645587) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.