Anti-JAM-A [6F4]

• Catalog Number: orb2645587
• Category: Antibodies
• Description: Anti-JAM-A [6F4]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6F4
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing BALB/c mice with MCF-7 cells. The humanized version was generated by grafting CDRs of parental mouse antibody onto human framework regions.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: JAM-A
• UniProt ID: Q9Y624
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD321; JAM-1; JAM1; JCAM; F11R; PAM-1; Junctional
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MCF7 cells with anti-JAM-A antibody 6F4. Immunofluorescence analysis of paraformaldehyde-fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 6F4 (orb2645587) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell junction staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645587, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-JAM-A antibody 6F4. Caco-2 cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of 6F4 (orb2645587) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.