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Anti-JAK2 (Phospho-Y221) Antibody

Catalog Number: orb665512

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb665512
CategoryAntibodies
DescriptionRabbit polyclonal antibody to JAK2 (Phospho-Y221)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIH, WB
ReactivityHuman, Mouse, Porcine, Primate, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y221 of human JAK2 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1000, IHC-P: 1-50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetJAK2
Entrez24514, 3717, 16452
UniProt IDQ62120, Q62689, O60674
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-Tyrosine-protein kinase JAK2 antibody, anti-J
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-JAK2 (Phospho-Y221) Antibody

Western blot analysis of JAK2 (Phospho-Y221) expression in HepG2 (A), LO2 (B) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 125 kD)

Anti-JAK2 (Phospho-Y221) Antibody

Immunohistochemical analysis of JAK2 (Phospho-Y221) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-JAK2 (Phospho-Y221) Antibody

Direct ELISA antibody dose-response curve using Anti-JAK2 (Phospho-Y221) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.