Phospho-JAK2 (Tyr221) Rabbit Polyclonal Antibody

• Catalog Number: orb6251
• Category: Antibodies
• Description: Phospho-JAK2 (Tyr221) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rat
• Reactivity: Human, Zebrafish
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human JNK2 around the phosphorylation site of Tyr221 IQD(p-Y)HI
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 131 kDa
• Target: JAK2
• UniProt ID: O60674
• RRID: AB_10922141
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: JAK2(phospho Y221); p-JAK2(phospho Tyr221); Tyrosi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HL-60, Primary Antibody (green line): Rabbit Anti-phospho-JAK2 (Tyr221) antibody (orb6251), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-JAK2 (Tyr221)) polyclonal Antibody, Unconjugated (orb6251) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-JAK2 (Tyr221)) Polyclonal Antibody, Unconjugated (orb6251) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.