Anti-Interleukin-6 (IL-6) Mouse

• Catalog Number: orb1125577
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Interleukin-6 (IL6)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS buffer, pH 7.4. Reconstitute in sterile water at a concentration of > 0.2 mg/ml.
• Purity: Affinity purified
• Purification: Affinity purified
• Immunogen: highly pure (>98%) recombinant mouse IL-6
• Tested applications: ELISA, WB
• Dilution range: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
• Application notes: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only