IL6 Antibody

SKU: orb345102

Description

IL6 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, Multiplex Assay, WB
• Dilution Range: ELISA: 1:10,000, FC: 0.5 mg/mL, WB: 1:1000
• Reactivity: Human
• Application Notes:
  Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 33A12.G9
• Immunogen: This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
• Target: IL6
• Purity: This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store IL 6 antibody at -20° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse Anti-IL-6 antibody, mouse anti-interleukin-6 antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF

Quick Database Links

Gene Symbol

IL6

UniProt

P05231

RefSeq (Protein):NP_000591.1