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Anti-IFN gamma Purified
Catalog Number: orb154408
| Catalog Number | orb154408 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against IFN gamma |
| Clonality | Monoclonal |
| Clone Number | 4S.B3 |
| Tested applications | ELISA, FC, ICC, IHC-P, IP, RIA, WB |
| Reactivity | Human, Primate |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Interferon gamma derived from human leukocytes |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | IFN gamma |
| Entrez | 3458 |
| UniProt ID | P01579 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti-IFG antibody, anti-IFI antibody, anti-IFN gam Read more... |
| Note | For research use only |
| Application notes | ELISA: This antibody is being used as detection antibody in combination with capture antibody NIB42. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Separation of human IFN gamma positive lymphocytes (red-filled) from IFN gamma negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).
