IFN gamma Antibody

SKU: orb154408

Description

Mouse monoclonal antibody against IFN gamma

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, IP, RIA, WB
• Reactivity: Human, Primate
• Application Notes:
  ELISA: This antibody is being used as detection antibody in combination with capture antibody NIB42. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 4S.B3
• Immunogen: Interferon gamma derived from human leukocytes
• Target: IFN gamma
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interferon gamma, IFN-gamma

Separation of human IFN gamma positive lymphocytes (red-filled) from IFN gamma negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Quick Database Links

Gene Symbol

IFN gamma

UniProt

P01579

NCBI Gene ID

3458