IL-2 Antibody

• Catalog Number: orb1562578
• Category: Antibodies
• Description: IL-2 Antibody
• Target: IL-2
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: recombinant human IL-2
• UniProt ID: P60568
• Tested applications: ELISA, FC, ICC, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.
• Clone Number: 35C3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin 2
• Note: For research use only
• Entrez: 3558

Flow cytometry multicolor intracellular staining of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood showing lymphocytes stained using anti-human CD4 (MEM-241) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

Separation of human CD4 positive IL-2 positive lymphocytes (red-filled) from CD4 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).