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Catalog Number | orb44869 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Human IgG (Fc). |
Clonality | Monoclonal |
Clone Number | EM-07 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Fusion protein of human IgG Fc fragment. |
Antibody Type | Primary Antibody |
Concentration | 0.1 mg/ml |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | IgG (Fc) |
RRID | AB_10991717 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-IgG (Fc) antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis (surface staining) of human peripheral blood using anti-IgG (Fc), clone EM-07, FITC.
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human IgG (Fc) (EM-07) FITC antibody (3 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human IgG (Fc) positive CD19 negative lymphocytes (red-filled) from CD19 positive IgG (Fc) negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) FITC antibody (3 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) FITC antibody (3 μl reagent / 100 μl of peripheral whole blood).