Goat Anti-Mouse IgG H&L, FITC conjugated

• Catalog Number: orb868188
• Category: Antibodies
• Description: Goat Anti-Mouse IgG H&L, FITC conjugated
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: FC, IF
• Reactivity: Mouse
• Immunogen: Native Mouse IgG
• Antibody Type: Secondary Antibody
• Concentration: 2.0 mg/ml
• Dilution range: IF=1:200-1000, Flow-Cyt=1:50-200
• Conjugation: FITC
• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Alternative names: Goat Anti-Mouse IgG H&L (FITC); Immunoglobulin G;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (orb500822) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (orb868188) for 90 minutes, and DAPI for nuclei staining.