HER3 Antibody

• Catalog Number: orb1088495
• Category: Antibodies
• Description: HER3 (ERBB3) is a transmembrane protein of the epidermal growth factor receptor family, although it does not have an active tyrosine kinase domain. It can bind its ligand, but for further signaling it needs heterodimerization with other receptor tyrosine kinases of EGFR family. Overexpression of HER3 has been observed in many carcinomas. Activity of HER3 can be modulated by one of its isoforms, that is secreted from the cell, as its lacks the transmembrane domain.
• Target: HER3
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: HER3-transfected CHO cells
• UniProt ID: P21860
• Tested applications: FC
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: H3Mab-17
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB3, FERLK, LCCS2
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 2065
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells stained using anti-HER3 (H3Mab-17) purified antibody (concentration in sample 0.6 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).