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CD86 Antibody

SKU: orb222751

Description

Mouse monoclonal antibody to CD86

Research Area

Epigenetics, Immunology, Infectious Diseases

Images & Validation

Tested ApplicationsFA, FC, IHC-Fr, IP, WB
ReactivityHuman
Application Notes
Functional application: The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2). Flow cytometry: Recommended dilution: 5 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.BU63
ImmunogenB-lymphoblastoid cell line ARH 77
TargetCD86
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

B7-2, FUN-1, LAB72

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CD86 Antibody

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD86 (BU63) purified antibody (low endotoxin, concentration in sample 3 μg/ml) GAM APC.

CD86 Antibody

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD86 (BU63) purified antibody (low endotoxin, concentration in sample 3 μg/ml) GAM APC.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
IP
Immunoprecipitation
View Protocol

CD86 Antibody (orb222751)

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0.1 mg
$ 280.00
DispatchUsually dispatched within 5-10 working days
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