CD79a Antibody

SKU: orb154414

Description

Mouse monoclonal antibody to CD79a which expressed in B-cells. Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3). The cytoplasmic tails of CD79A contains an ITAM (immuno-receptor tyrosine-based activation) motif, which acts to initiate the BCR signaling reactions by binding to and activating tyrosine

Images & Validation

• Tested Applications: FC, IHC-P, IP, WB
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Human, Mouse, Porcine, Primate, Rabbit, Rat
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml, intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: HM47
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Target: CD79a
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

BCR alpha, Ig-alpha, MB-1, IGA

Separation of human CD79a positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD79a (HM47) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD79a (HM47) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Western blotting analysis of human CD79a using mouse monoclonal antibody HM47 on lysates of Raji and Jurkat (negative control) cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD79a was detected around 43 kDa.

Quick Database Links

Gene Symbol

CD79a

UniProt

P11912

NCBI Gene ID

973