CD79a Antibody

• Catalog Number: orb1088504
• Category: Antibodies
• Description: CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
• Target: CD79a
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: IgM complex isolated from Daudi cells
• UniProt ID: P11912
• Tested applications: ELISA, FC, ICC, IHC-P, IP
• Application notes: Flow cytometry: Recommended dilution: 1-5 µg/ml.
• Antibody Type: Primary Antibody
• Clone Number: ZL7.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BCR alpha, Ig-alpha, MB-1, IGA
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 973

Separation of lymphocytes stained anti-human CD79a (ZL7/4) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD79a (ZL7/4) purified antibody (concentration in sample 5 μg/ml, GAM APC).