Anti-Hu CD5 Purified

• Catalog Number: orb43737
• Category: Antibodies
• Description: Mouse Monoclonal to CD5.
• Clonality: Monoclonal
• Clone Number: CRIS1
• Tested applications: ELISA, FC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: stimulated human leukocytes
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD5
• Entrez: 921
• UniProt ID: P06127
• RRID: AB_10993526
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD5 antibody
• Note: For research use only
• Application notes: ELISA: The antibody CRIS1 can be used in the sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: Jurkat human leukemia T-cell line, HPB human leukemia T-cell line.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of human peripheral blood lymphocytes (PBL) stained with anti-human CD5 (CRIS1) antibody (blue; dilution of purified antibody 1 µg/ml); overlay with mouse IgG2a isotype control (red).

Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.