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Item 1 of 3
Anti-Hu CD47 Purified
Catalog Number: orb44051
| Catalog Number | orb44051 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD47. |
| Target | CD47 |
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Conjugation | Unconjugated |
| Reactivity | Human, Porcine, Primate |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Immunogen | COS-7 (African green monkey) cells |
| UniProt ID | Q08722 |
| RRID | AB_10996765 |
| Tested applications | FC, IHC-Fr, WB |
| Dilution range | Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions. |
| Application notes | Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions. |
| Antibody Type | Primary Antibody |
| Clone Number | MEM-122 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-CD47 antibody, anti-Integrin Associated Prote Read more... |
| Note | For research use only |
| Entrez | 961 |
Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. orb43568); Panel B - porcine PBMC sample stained with anti-CD47 (MEM-122). Cells in the granulocyte gate were used for analysis.
Separation of human lymphocytes (red-filled) from human CD47 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).
