CD47 Antibody

SKU: orb44051

Description

Mouse Monoclonal to CD47.

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: FC, IHC-Fr, WB
• Reactivity: Human, Porcine, Primate
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: MEM-122
• Immunogen: COS-7 (African green monkey) cells
• Target: CD47
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

IAP, OA3, MER6

Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. orb43568); Panel B - porcine PBMC sample stained with anti-CD47 (MEM-122). Cells in the granulocyte gate were used for analysis.

Separation of human lymphocytes (red-filled) from human CD47 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722

NCBI Gene ID

961