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Description
Research Area
Images & Validation
−| Tested Applications | FC, IP |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Clone No. | MEM-16 |
| Immunogen | HPB cell line (human peripheral blood leukemia T-cells) |
| Target | CD4 |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Separation of human CD4 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD4 (MEM-16) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (MEM-16) purified antibody (concentration in sample 4 μg/ml, GAM APC).
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Protocol Information
CD4 Antibody (orb44114)
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