CD34 Antibody (FITC)

SKU: orb154484

Description

Mouse monoclonal antibody conjugated to FITC which recognizes CD34 that is a heavily glycosylated member of the CD34 family. It expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It could play role as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 may also play a role in adhesion of certain antigens to endothelium.

Research Area

Cardiovascular Disease, Epigenetics, Neuroscience, Stem Cells

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 581
• Target: CD34
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD34 (581) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (581) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using anti-human CD34 (581) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD34

UniProt

P28906

NCBI Gene ID

947