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Catalog Number | orb461547 |
---|---|
Category | Antibodies |
Description | CD34 Rabbit Polyclonal Antibody (FITC) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF |
Predicted Reactivity | Bovine, Canine, Porcine, Rabbit, Rat |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human CD34 (301-385/385aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IF=1:200-800 |
Conjugation | FITC |
Target | CD34 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | CD34 antigen; CD34 molecule; Cluster designation 3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-CD34 antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-CD34 Antibody, conjugated (orb461547) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
IHC, WB | |
Canine, Goat, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
FITC |